The twin spot generator for differential Drosophila lineage analysis., Sustar Anne, Bonvin Marianne, Griffin Ruth, Binari Richard, del Valle Rodriguez Alberto, Hohl Amber M, Bateman Jack R, Villalta Christians, Heffern Elleard, Grunwald Didier, and Bakal Chris, Nature methods, 2009 Aug, Volume 6, Issue 8, p.600-2 (2009)
In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies.